Phage Display
Phage display is an antibody discovery technology that inserting the DNA sequence of foreign protein or peptide into the appropriate position of phage coat protein structural gene, so the exogenous gene can be expressed and displayed on the surface of phage. By applying this technology, phage libraries can be established to express a wide diversity of peptides or proteins and can be employed to select those binding to the desired targets.
Feature
- Wildely-used format: scFv (single chain variable fragment), which can be utilized alone or further. transformed to fusion protein or monoclonal antibody.
- High diversity: AcadeMab’s scFv library is cloned from 572 individual.
- Fast targeting: single run screening can be completed in 15 days.
- Animal-free: whole process can be conducted without animal sacrificing.
Application:
- The selected scFv or transformed fusion protein or monoclonal antibody can be applied for the therapeutics or diagnostics purpose.
- The selected scFv can be used as a part of antibody drug conjugate (ADC) or cell therapy by its targeting function.
- Phage display library can be used to screen antibodies that recognize toxicity or autoantigens, which would harm the animals during the immunization.
- Target specific library can be established for antibody affinity maturation or epitope mapping.
Status:
The standardized procedure for this technology platform is well established. The options of standardized/customized fee-for-service or co-development are open for discussion.